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VA-SOP-LC-006 · Standard operating procedure
Reprocessing data files with a new method in LCsolution Postrun Batch
SOP with screenshots: reprocess all injections with a corrected integration method using the LCsolution Postrun Batch Table, without re-injecting.
| SOP No. | VA-SOP-LC-006 |
|---|---|
| Revision | 00 |
| Effective date | 30 October 2026 |
| Review date | 30 October 2027 |
| Software | Shimadzu LCsolution 1.25 (Postrun Analysis), LC-2010 series HPLC |
1Purpose
To apply a revised data-analysis method to existing data files in one step using the Postrun Batch Table.
2Scope
Applies to LCsolution 1.2x Postrun Batch Processing.
3Responsibility
- Analyst — performs the procedure and records results.
- QC reviewer — verifies the output against the acceptance criteria.
- Lab in-charge / system administrator — controls method and report-format files and user rights.
5Prerequisites and materials
- Revised method file (.lcm) saved.
- Data files to reprocess, preferably copies.
6Safety and precautions
- Work on a copy of data and method files until the change is approved.
- Never delete or overwrite raw data (.lcd) to obtain a passing result; every change must be traceable.
- Follow the laboratory's data-integrity and audit-trail SOP when LCsolution security is enabled.
7Procedure
-
7.1
Set the method in the Batch Table
Postrun → Batch Processing: put the new method (e.g. VA_Caffeine.lcm) in the Method File column; Edit → Fill Down copies it to every row.
Fig. 7.1 — Set the method in the Batch Table -
7.2
Start the batch
Click Batch Start. If a data file is open, the "Close Read File" prompt appears — click OK.
Fig. 7.2 — Start the batch -
7.3
Check completion
All six data files are reprocessed in seconds.
Fig. 7.3 — Check completion -
7.4
Open one injection
Double-click any file, e.g. Caffeine_04.
Fig. 7.4 — Open one injection -
7.5
Verify the new integration
Impurity separated, main peak Area % 82.7.
Fig. 7.5 — Verify the new integration -
7.6
Good practice
Work on a copy; File → Rollback to Original Data gives a clean start.
8Acceptance criteria and interpretation
- Every row of the batch shows the new method and finishes without error.
- Opened data files show the new integration (e.g. impurity separated, Area % 82.7).
9Troubleshooting and common errors
| Symptom | Cause and fix |
|---|---|
| "Close Read File" message | A data file is open in another window. Click OK; LCsolution closes it first. |
| Need the original result back | File → Rollback to Original Data restores the acquisition-time processing. |
10References
- Shimadzu LCsolution Ver. 1.2x — Operation and Help documentation.
- USP <621> Chromatography.
- ICH Q2(R2) Validation of Analytical Procedures.
11Revision history
| Rev. | Date | Change |
|---|---|---|
| 00 | 30 October 2026 | First issue, written from the October 2026 screen recording. |
Frequently asked
How do I reprocess HPLC data in LCsolution without injecting again?
Put the new method in the Method File column of the Postrun Batch Table (Edit → Fill Down) and click Batch Start.
Video version: LCsolution — Changed your integration method? Reprocess all six injections (October 2026) — on our Instagram and YouTube.
Related SOPs
Vision Analytical is an independent service provider, not affiliated with Shimadzu Corporation or Waters Corporation. LCsolution and Empower are trademarks of their respective owners. Screenshots are from our own demonstration systems with demonstration data. Last updated 30 October 2026.